TCO* for labelling neuronal compartments

Ivana Nikić-Spiegel et al. use Trans-Cyclooct-2-en-L-Lysine (TCO*; SC-8008) to label certain parts of neuronal compartments (axon initial segment (AIS)). Together with a clickable dye, these become visible under the microscope. The developed method is particularly suitable for labelling complex and spatially limited proteins.

Journal of Cell Science (2023) 136: Direct fluorescent labeling of NF186 and NaV1.6 in living primary neurons using bioorthogonal click chemistry