In Würzburg, experienced scientists and young researchers from all over the world come together to discuss the state of the art regarding the use of genetic modifications (e.g. genetic code expansion) for future biomedical treatments.

The Translational Bioimaging Symposium 2022

Here, bioorthogonal click chemistry plays a key role – SiChem offers a wide range of useful tools for this kind of chemical reactions.

Click Chemistry Tools

Milan Vrabel et al. believe that performing abiotic chemical reactions within particular organelles at the subcellular level opens up new avenues: by manipulating biological processes in a targeted manner, they can be studied in more detail and this strategy can also be used to develop organelle-targeted drug-delivery systems. (TCO-NHS / SC-8070 / SC-8072 / SC-8073)

Rastislav Dzijak et al.: JACS Au 2021, 1, 23−30 : Structurally Redesigned Bioorthogonal Reagents for Mitochondria-Specific Prodrug Activation

https://dx.doi.org/10.1021/jacsau.0c00053

Gerti Beliu et al. from the University of Würzburg use SiChem’s unnatural amino acid Trans-Cyclooct-2-en-L-Lysine (TCO*A; SC-8008 labelled with tetrazine dyes) for dSTORM experiments. A complete labelling and imaging pipeline has been developed to visualize transmembrane proteins in living neurons.

Diogo Bessa-Neto et al. : NATURE COMMUNICATIONS (2021) 12: 6715: Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids unveils masked epitopes in live neurons

https://doi.org/10.1038/s41467-021-27025-w

Neutrophils are often made responsible for killing bacteria in chronic diseases such as cystic fibrosis (CF). Here, however, there is an unexplained phenomenon: although neutrophils are massively present, long-lasting bacterial infections occur at the same time. Magaroli et al. from Emory University are trying to get to the bottom of this phenomenon and are also measuring the activity of free extracellular neutrophil elastase (NE) using SiChem’s FRET-based NEmo-1 probes (SC-0200 / SC-0201).

Margaroli et al., 2021, Cell Reports Medicine 2, 100239: Transcriptional firing represses bactericidal activityin cystic fibrosis airway neutrophils

https://doi.org/10.1016/j.xcrm.2021.100239

Fluorescent probes that react with complementary bioorthogonal reagents and subsequently light up are excellent tools for bioimaging applications.

Vrabel et al. have synthesized a series of diverse coumarin-tetrazine probes that can react with SiChem’s TCOs and BCNs in seconds to act as labels in living cells. (SC-8106 / SC-8406)

 

Juraj Galeta, et al.: A Systematic Study of Coumarin–Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging

Chem. Eur. J. 2020, 26, 9945 – 9953

https://doi.org/10.1002/chem.202001290

Bremen is rather known as a trading city – less as a high-tech location for internationally operating biotech companies. But there are quite a few innovative, research-based companies that offer their products and services worldwide. Some of them are now presented by the Wirtschaftsförderung Bremen – SiChem is one of them ! Stories about Bremen Business

SiChem receives BMWi grant (ZIM) together with TU Clausthal for developing Smart Simulated Moving Bed Chromatography (sSMB)

Personalised medicine is about treating patients as individually as possible. The drugs administered should have an optimal effect. Often, the patient is given precisely tailored treatment strategies with several drugs that work in combination. However, this also means that there will be fewer and fewer blockbusters that are produced and used in large batches. In the long term, we will see an increase in the number of drugs that have to be produced in smaller batch sizes but with the same quality requirements as large batches.

The production of such high-purity products is complex and is often achieved by batch chromatography as a selective and efficient technique. Process design and up-scaling are relatively simple. However, good separation performance is offset by high product losses, relatively low productivity and, above all, high solvent consumption.

This can be changed by continuous chromatography processes: productivity increases by a factor of 3 to 30 and solvent reduction by a factor of 10 are possible.

Currently, there is a number of available techniques, starting with Simulated Moving Bed Chromatography (SMB), through various sequential multi-column connections, to more specialized processes such as Multicolumn Countercurrent Solvent Gradient Purification (MCSGP)

Each process offers its own advantages and areas of application, but also limitations. In common with the continuous processes is that they deliver very high product quality at high throughput. However, the complexity of several techniques with up to 8 chromatography columns and 5 gradient pumps is very high. These separation techniques are therefore expensive and rarely used in small research-based pharmaceutical companies.

In this project, a simplified smart SMB (sSMB) shall be developed that requires only one column but still uses several adsorbents.

 

Dear customers and partners,

we are all part of a complex supply chain in a globalized world, with all its advantages and disadvantages.

We are currently experiencing the rapid global spread of COVID-19 and would like to take responsibility as a supplier: most importantly, SiChem has taken all necessary preventive measures, e.g. home office where possible. In this way we want to ensure that our employees and their families remain healthy. Our employees will continue to support our customers with full commitment, either at the production site or from the home office.

We are convinced that we will master this situation together !

Stay healthy,

the SiChem team

The conference will take place from 12th to 15th of December at the Oregeon Health and Science University (OHSU) in Portland, USA.
This biennial international conference focuses on the growing intersection of chemical biology and physiology. This exciting field offers innovative opportunities for the development of drug targets and therapy concepts. Leading scientists from all over the world meet here to promote collaboration, and stimulate cutting-edge research. This year’s focus is on chemical physiology, imaging techniques, glycochemical biology, nucleotide chemistry and optical tools.

We will be at the CPhI in Frankfurt on November 6th and we still have available dates – if you  like to meet us, just send us an e-mail and let us know when you will be available (info@sichem.de).