In order to study identity, function, location, transport and interacting proteins of lipids, trifunctional lipid (TFL) derivatives provide the ideal tool to answer your research questions. The combination of three functional groups allows for excellent temporal control of lipid release through uncaging, protect from wash-out in fixed samples and covalent connections to binding proteins through orthogonal photo-crosslinking, and isolation or fluorescent tagging of protein-lipid conjugates through click chemistry.

 TFLs are ideally suited to study

• intracellular lipid localization
• lipid−protein interactions
• lipid metabolism

with a single tool, that combines a photo-removable protecting group (cage), a photo-crosslinkable diazirine group and a terminal alkyne group useful for click chemistry in live cells.

Flash & Click Tools you will find here

Alkyl diazirines are photo-reactive functional groups frequently used in photo-affinity labeling (PAL). They are compact in size, being nearly isosteric to a methyl group, and they are activated at a wavelength of light (~355 nm) that is not damaging to protein(s). Photo-crosslinked protein targets are then visualized by the reporter group (e.g. fluorophore, biotin, or radioactive label). Covalent bond formation between the probe and targets enable the subsequent purification and identification of the targets using techniques such as SDS-PAGE, immunoprecipitation, biotin-streptavidin affinity purification and mass spectrometry. Alkyl diazirines are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark.

New photocrosslinking compounds you will find here

 

The first rapidly reversible chemical dimerization system – rCD1 – , which permits to determine kinetics of lipid metabolizing enzymes in living cells, is now available at SiChem . This new system was applied to induce and stop the activity of phosphatidyl 3-kinase (PI3K) (SC-7000_Chemical_Dimerizer_info). rCD1 is available in 2 sizes: 100µg (for app. 20 experiments) and 500µg (SC-7000)

A representative time-lapse fluorescence movie of HeLa cells co-transfected with NLS2-SNAPf-ECFP and mRFP-FKBP constructs. rCD1 (1 μM) induces translocation of mRFP-FKBP into the nucleus; addition of FK506 (1 μM) leads to release of mRFP-FKBP from the nucleus !