The first rapidly reversible chemical dimerization system – rCD1 – , which permits to determine kinetics of lipid metabolizing enzymes in living cells, is now available at SiChem . This new system was applied to induce and stop the activity of phosphatidyl 3-kinase (PI3K) (SC-7000_Chemical_Dimerizer_info). rCD1 is available in 2 sizes: 100µg (for app. 20 experiments) and 500µg (SC-7000)

A representative time-lapse fluorescence movie of HeLa cells co-transfected with NLS2-SNAPf-ECFP and mRFP-FKBP constructs. rCD1 (1 μM) induces translocation of mRFP-FKBP into the nucleus; addition of FK506 (1 μM) leads to release of mRFP-FKBP from the nucleus !

Chemical_Dimerizer_Movie

 

 

 

 

INO-4995 (1-O-octyl-2-O-butyryl-D-myo-inositol 3,4,5,6-tetrakisphosphate octakis(propionoxymethyl)ester) is an ion channel modulator that is slowly metabolized in cells. Studies have shown that it inhibits ENaC activity while promoting expression of the calcium-activated chloride channel, TMEM16a, in membranes. Thus it has potential therapeutic activity in the treatment of cystic fibrosis where it normalized nasal potential difference (npd) in a murine CF knock-out model and promoted fluid secretion in human CF airway epithelia.

Strong preclinical efficacy of INO-4995: a membrane-permeant Inositol-phosphate derivative has potential in treatment of Cystic Fibrosis (CF)
This graph is showing the difference in npd between mice before and after 4-day treatment with INO-4995 (0.0008mg/kg):
Am J Respir Cell Mol Biol 2010, 42(1), 105

 

The competition ” 100 Landmarks in the Land of Ideas” promotes ideas for urgent issues of tomorrow. The i3 – Life Sciences Cluster tries to find answers to the increasing life expectancy and the associated diseases. Innovative diagnostic companies in the Bremen / Bremerhaven region have joined forces to investigate biomarkers that may indicate certain diseases more accurately.

 

                                                                                                  Fotograf. G. Ketschau (BIS, Bremerhaven)